Production and bioprocessing of Taxol from Aspergillus niger, an endophyte of Encephalartos whitelockii, with a plausible biosynthetic stability: antiproliferative activity and cell cycle analysis

The biosynthetic potency of Taxol by fungi raises their prospective to be a platform for commercial production of Taxol, nevertheless, the attenuation of its productivity with the fungal storage, is the challenge. Thus, screening for a novel fungal isolate inhabiting ethnopharmacological plants, with a plausible metabolic stability for Taxol production could be one of the most affordable approaches. Aspergillus niger OR414905.1, an endophyte of Encephalartos whitelockii, had the highest Taxol productivity (173.9 μg/L). The chemical identity of the purified Taxol was confirmed by HPLC, FTIR, and LC–MS/MS analyses, exhibiting the same molecular mass (854.5 m/z) and molecular fragmentation pattern of the authentic Taxol. The purified Taxol exhibited a potent antiproliferative activity against HepG-2, MCF-7 and Caco-2, with IC50 values 0.011, 0.016, and 0.067 μM, respectively, in addition to a significant activity against A. flavus, as a model of human fungal pathogen. The purified Taxol displayed a significant effect against the cellular migration of HepG-2 and MCF-7 cells, by ~ 52–59% after 72 h, compared to the control, confirming its interference with the cellular matrix formation. Furthermore, the purified Taxol exhibited a significant ability to prompt apoptosis in MCF-7 cells, by about 11-fold compared to control cells, suppressing their division at G2/M phase. Taxol productivity by A. niger has been optimized by the response surface methodology with Plackett–Burman Design and Central Composite Design, resulting in a remarkable ~ 1.6-fold increase (279.8 μg/L), over the control. The biological half-life time of Taxol productivity by A. niger was ~ 6 months of preservation at 4 ℃, however, the Taxol yield by A. niger was partially restored in response to ethyl acetate extracts of E. whitelockii, ensuring the presence of plant-derived signals that triggers the cryptic Taxol encoding genes.


Introduction
Cancer diseases are currently ranked as the second notable reason of mortality after cardiovascular disease [1,2].Chemotherapy has been recognized as one of the most profound treatment protocol for cancer, and Taxol is one of the most prescribed drugs.Taxol, a diterpenoid that was initially isolated from Taxus brevifolia with a significant anticancer activity by arresting the tumor cell cycle at the G2/M phase [3,4].Taxol had a powerful anticancer activity against a wide-range of tumor cell lines including breast, ovarian, hepatic, leukemia, lung tumors, and polycystic kidney disease [5,6].The influential activity of Taxol against various tumor cells is mainly due the unique affinity to bind with N-terminal region of β-tubulin, stopping the depolymerization of the microtubules, so, blocking the mitotic division, with an ultimate arrest to the cell cycle of tumor cells [7,8].Taxol was originally derived from T. brevifolia bark, nonetheless, its significantly low yield (~ 0.001%), unpredicted fluctuations and reproducibility, due to the natural environmental changes that could have a negative effect on the Taxol biosynthetic machinery by T. brevifolia, are the challenge for this approach [9,10,11].Semisynthetic technology based on Taxus baccata for production of the intermediate 10-decaetylbaccatin III, followed by tailoring enzymes to synthesize Taxol, has been used as an authenticated approach for the commercial Taxol production [12], however, the heterogeneity of these intermediates are the major obstacle.Interestingly, the fungal metabolic potency of Taxol production raise the hope for implementation of this approach commercially, for the feasibility of manipulating their yield, independence on the environmental conditions, rapid growth, and cost-effectiveness [13,14].Numerous endophytic fungi were reported to possess the Taxol producing metabolic potency [15][16][17][18][19][20].The biosynthetic pathway of Taxol in fungi started with cyclization of geranylgeranyl diphosphate to taxa-4(5),11 (12)-diene by the action of taxadiene synthase, then hydroxylation of taxadiene nucleus by the cytochrome P450-monooxygenases, as reviewed in details by our previous studies [21].However, the anticipation of fungi to be a commercial approach for Taxol production has been confronted by the attenuation of Taxol productivity by fungi with the storage and multiple subculturing [16,[21][22][23][24].The machinery of Taxol biosynthesis in fungi is usually encoded by gene cluster located on different domains on the fungal genome, and the expression of these cluster become cryptic under standard lab conditions, due to the lack or dilution of the transcriptional signals to synchronize the expression of these genes [18,[24][25][26][27][28].Thus, screening for a metabolically stable Taxol producing fungal isolate from different medicinal plants is the objective.
Medicinal plants of ethno-pharmaceutical uses could be a repository for novel endophytic fungi with unique metabolic stability and sustainability for bioactive secondary metabolites.The genus Encephalartos has been recognized as one of the well-known pharmaceutically valuable plants, the second-largest genus of the family Zamiaceae, predominantly developed in south of the Sahara Desert [29][30][31], tropical Africa, over 50% of the genus has been present in South Africa [32].Encephalartos has a significant ethno-pharmacological value, exhibiting cytotoxic and antifungal properties [33][34][35], however, the identity of the endophytic fungi from this genus remains ambiguous.Thus, the objective was to search for a potential endophytic fungal isolate from Encephalartos whitelockii, with a reliable biosynthetic stability for Taxol production, and to maximize the yield of Taxol via Surface Response Methodology optimization bioprocessing.

Plant sample collection, and isolation of the fungal endophytes
Fresh leaves samples of Encephalartos whitelockii were obtained in March 2022 from El-Abd Garden in Giza, Egypt, for isolation of their endogenous fungal endophytes.The plant was kindly identified by Dr. Therese Labib, a Plant Taxonomist at Orman Botanical Garden in Giza, Egypt, and the sample voucher was deposited at the Department of Pharmacognosy Herbarium, Faculty of Pharmacy, Zagazig University, with ID# ZU-Ph-Cog-608 was deposited.The leaves were gathered and sterilized by 70% ethanol for 2 min, then by 2.5% sodium hypochlorite for 2 min, rinsed twice with distilled water.The leaves were carefully segmented into 1 cm × 1 cm, and placed onto the surface of potato dextrose agar (PDA) [20] (200 g/L potato extract, 20 g/L glucose, and 20 g/L agar in distilled water, with 0.1 g of chloramphenicol).The plates were incubated at 30 ℃ for 10 days [16,36], and the emerged colonies of fungi were purified on the same media, and the developed fungi were identified according to their morphological features [37][38][39]

Screening for Taxol production by the endophytic fungi
The endophytic fungi from the leaves of E. whitelockii were grown on potato dextrose broth (PDB) [16,20].A plug of 6-day-old culture on PDA of each isolate was inoculated into PDB medium, the cultures were incubated at 30 ℃ for 10 days, filtered, the filtrates were centrifuged at 5000 rpm, followed by extraction with ethylacetate [40,41].The collected organic phase of solvent was dried by rotary evaporation, the resulting residues were dissolved in methanol.The Taxol productivity was evaluated by Merck 1 mm (20 × 20 cm) pre-coated silica gel TLC plates (Silica gel 60 F254, KGaA, Darm, Germ.) [20,42], with methylene chloride/ methanol/dimethyl formamide of 90:9:1 (v/v/v).The TLC plates were UVillumined at λ 254 nm, to visualize the putative Taxol spots, as well as the plates were sprayed with a 1% vanillin sulfuric acid solution, and gently heated for 24 h.The putative Taxol sample gave the same bluish color and mobility rate of the authentic one (Cat.# T7402), were considered.The putative silica spots containing Taxol were scraped off for Taxol extraction [16,20,21].The concentration and purity the extracted Taxol were evaluated using HPLC (Agilent Technology, G1315D) of Eclipse Plus RP-C18 column (Cat.# 959963-902) with methanol/ acetonitrile/ water (25:35:40, v/v/v) at 1.0 mL/min for 20 min.The fractions of Taxol were scanned using a photodiode array detector (DAD) from 200 to 500 nm, and the concentrations of the putative Taxol were determined, compared to the authentic one at λ 227 nm [20,43].

Spectroscopic analyses of Taxol
The absorbance of the putative Taxol from the most potent fungal isolate was measured by UV-Vis spectrophotometry at wavelength range 200-400 nm.The concentration and purity of the extracted sample were assessed, compared to the authentic Taxol, with methanol for zeroing the spectrophotometer [16].The Infra-Red (FT-IR) spectra of the sample were determined using a JASCO, FTIR 6100 Spectrophotometer, sample was pulverized in KBr pellets, and the spectra were recorded from 4000 to 500 cm −1 .
The identity of the purified Taxol was resolved by the LC-MS/MS with a Thermo Scientific LCQ Deca mass spectrometer and Hypersil Gold aQ (C18 column) equipped with a positive ion mode electrospray source.The gradient elution mobile phase system of solution A (0.1% formic acid) and B (acetonitrile in 0.1% formic acid), was used with at 0.2 mL/min for 40 min, with a mobile phase B gradient ranging from 2 to 98% [16,17,21].The chemical features of the committed signals were assessed by analyzing their fragmentation pattern with the NIST mass spectral library.

Molecular identification of the potent Taxol-producing fungal isolate
The potent Taxol-producing fungal isolate was molecularly identified based on the internal transcribed spacers (ITS) sequence [44,45].The fungal genomic DNA (gDNA) was extracted by CTAB reagent [46], used as a PCR template with the primers ITS5 5ʹ-TCC TCC GCT TAT TGA TAT GC-3ʹ and ITS4 5ʹ-GAA GTA AAA GTC G TAA CAA GG-3ʹ [47].The reaction of PCR contains 10 µL of 2X TOPsimple ™ DyeMIX-nTaq (Cat.# P510T), 1 µL gDNA, 1 µ of each primer (5 pmol) in 20 µL total volume.The PCR program was denaturation at 95 ℃ for 2 min, followed by 35 cycles of denaturation at 95 ℃ for 30 s, annealing at 55 ℃ for 40 s, and extension at 72 ℃ for 40 s, and then 72 ℃ for 2 min in final extension.The PCR amplicons were analyzed by 1.5% agarose gel, and the amplicons were purified, sequenced, and the obtained sequences were non-redundant BLAST searched, then imported into MEGA X software, and aligned by the ClustalW algorithm.Finally, the phylogenetic analysis was assessed using the neighbor-joining with 100 bootstrap replicates [48].

Antiproliferative activity of the crude ethylacetate and purified Taxol extracts
The anticancer activity of the crude ethyl acetate extract, and purified Taxol of the potent fungal isolate towards the human hepatocellular (Hep-G2), breast (MCF-7) and intestinal (Caco-2) carcinoma, compare to the Vero cells was assessed by MTT assay [49].The 96-well microtiter plate was initially inoculated with 2 × 10 3 cells/ well, incubated at 12 h at 37 ℃, then ethyl acetate and purified Taxol were added to at different concentrations, then further incubated of the plate for 48 h at 37 ℃ at 4% CO 2 .The MTT reagent was added to the plate, incubated for 6 h, the resulting purple formazan compound was assayed at λ 570 nm.The IC 50 values were represented by the concentration of Taxol inhibits the cell growth by 50%, compared to the control group (without drug).For the normal cell line (Vero), the CC 50 value was determined by the drug concentration reducing the initial cellular growth by 50%.The selectivity index (S.I.) was expressed by the value of CC 50 of the Vero cell to the IC 50 value towards the cancer cells.

Biological activity-guided assay of ethylacetate extract and putative Taxol extracts
The antifungal activity of ethyl acetate extract of the potent fungi and the putative purified Taxol sample was evaluated against Aspergillus flavus, as a model human fungal pathogen [50,51].The fungal spores was mixed with PDA prior plate pouring, incubated for 6 h, then different concentrations (0.1, 0.5, 2.5, 5, and 10 µg/mL) of the extracted Taxol were injected to the wells of the plate cultures, incubated at 28 ℃ for 5 days, and the diameter of the fungal inhibition zones was determined.The negative control was 1% DMSO.Triplicates of the experiments were performed, and the results were presented by the mean ± standard deviation.Minimum inhibitory concentration (MIC) was assessed as the lowest extract concentration effectively inhibits the visual fungal growth, based on the inhibition zone diameters [52].

Anti-wound healing activity of the extracted Taxol
The effect of purified Taxol from the potent fungus on the stopping the cellular migration of MCF-7 and HepG-2 tumor cells was determined.The 12-wells microtiter plate were seeded with at 4 × 10 6 cells to, allowed to grow to a confluent monolayer, then a wound was made on the cell layer, the plate was rinsed with PBS, and amended with fresh medium with the extracted Taxol at IC 25 value, compared to 2% DMSO as a negative control.The culture was incubated at 37 ℃ at 4% CO 2 , and the wound closure were monitored and imaged by phase-contrast microscopy.The healing efficiency of wound was calculated the percentage of a scratch area in drug-treated cells, compared to untreated cells.

Apoptosis and cell cycle analyses of the MCF-7 cells due to the putative Taxol
The apoptotic analysis of MCF-7 cells was assessed by Annexin-Apoptosis Detection Kit (Catalog #: K101-25).This assay based on the reaction of Annexin V protein with the externalized phosphatidylserine (PS) of the cells, with the initial stages of apoptosis, and the complex of Annexin V-PS can be easily measured by flow cytometry [53].The MCF-7 cells were seeded to a 96-well plate culture at 2 × 10 7 cells/well, treated with the purified Taxol, incubated for 24 h.The cultured cells were harvested, washed with 1 mL of PBS, and 200 μL of 1X annexinbinding buffer was added.After the addition of Annexin V-FITC and PI to the cells, the mixture was incubated in total darkness for 15 min.The Annexin-PS complex was detected (Ex, λ 488 nm; Em, λ 530 nm) using a FITC signal detector.
The analysis of MCF-7 cell cycle in response to purified Taxol was assessed by propidium Iodide (PI) assay Kit (Cat #. ab139418).Briefly, the 48-wells microtiter plate were seeded with the cells, incubated for 12 h at 37 ℃, Taxol was added at IC 25 value, and then the cultures were incubated for 48 h.The cells were harvested by centrifugation for 5 min at 2000 rpm, fixed in ice-cold 70% ethanol for 2 h at 4 ℃, and then the cells were rehydrated with 1 mL of PBS, and stained with PI solution containing 5 μg/mL RNase, for 30 min in dark.Subsequently, the cellular DNA were analyzed using flow cytometry, with excitation at λ 493 nm and emission at λ 636 nm, using Flow Jow software package for calculating the of G0-G1, S, and G2-M cells percentages.

Nutritional bioprocessing of A. niger for optimizing the Taxol yield by plackett-burman and central composite designs
Various nutritional requirements including maltose, sucrose, lactose, peptone, soytone, yeast extract, ammonium tartrate, sodium acetate, sodium nitrate, calcium chloride, magnesium sulfate, potassium hydrogen phosphate, ammonium sulfate, fluconazole, methyl jasmonate, cysteine, phenylalanine, methionine and glycine, were optimized by Plackett-Burman design for maximizing the Taxol yield of A. niger.The nineteen parameters were symbolized by (+ 1) and (− 1), as high and low levels, respectively.Response Surface Methodology has been recognized as an effective approach for evaluating the interactions of the independent parameters and their consequences on productivity of Taxol by fungi [40].The first-ordered polynomial model was calculated from the determination coefficient (R2), and F-test according to the following equation: Y is predicted yield of Taxol, Xi an independent variable, βi the linear coefficient, and β0 the model intercept.Three replicates were performed for each trial.The most influential variables derived from the Plackett-Burman Design (PBD), influencing on the Taxol production by A. niger were optimized by the Central Composite Design (CCD) [44,83].
The second-ordered polynomial model has been utilized for predicting the optimum storage conditions for Taxol production according to the following equation: β i the variables regression coefficient, β ii the regression coefficient of square effects, β ij the regression coefficient of interactions.

Taxol productivity by A. niger with the storage, potency of restoring their productivity by the plant extracts
The metabolic biosynthetic stability of Taxol by A. niger, with the storage was assessed.The original fungal culture was preserved as slope cultures at 4 ℃ for 10 months, and the Taxol productivity by the fungus was monthly assessed by growing at the standard conditions, then Taxol was extracted and quantified by the TLC and HPLC [14,21].
The impact of different extracts of E. whitelockii namely; dichloromethane, ethyl acetate, and ethanol on induction of the Taxol productivity by A. niger was assessed.The fresh leaves (5 g) of E. whitelockii were minced in 50 mL of each solvent, and the mixture was stored at 4 ℃ for 12 h, and the resulting extracts were filtered, centrifuged at 5000 rpm for 10 min, and the extracts were concentrated to 10 mL.Different concentrations of the prepared extracts were added to the 5-days old pre-cultures of A. niger, and further incubated for 14 days, and then Taxol was extracted and quantified [36].Blank media without A. niger, with the plant extracts, and control cultures of A. niger without plant extracts, were used.

Fungal deposition
The internal transcribed sequence of A. niger, an endophyte of E. whitelockii, was deposited to Genbank with accession number OR414905.1.

Statistical analysis
Triplicates of each experiment were executed, and the results were represented by the mean ± standard deviation.The significance and F-test statistical analysis was determined the one-way ANOVA by using Prism Version 6.0 (GraphPad Software, Inc., CA, USA).

Isolation of the endophytes of Encephalartos whitelockii, screening for Taxol production
Eight fungal endophytes were recovered from the leaves of E. whitelockii on PDA media, these isolates were morphologically identified based on their macroscopic and microscopic traits into four genera Aspergillus, Penicillium, Cladosporium and Fusarium (Fig. 1).Among the recovered genera, Aspergillus was represented by 55%, A. niger, A. awomari, A. candidus, A. flavus and A. fumigatus.Taxol productivity by the recovered endophytes of E. whitelockii were assessed on PDB, incubated at standard conditions, then Taxol was extracted and quantified.From the results (Fig. 1), the maximum Taxol yield was reported for A. niger (173.9 μg/L), followed by Fusraium solani (55.2 μg/L), A. candidus (40.2 μg/L) and A. awomari (35.2 μg/L).However, the other fungal isolates had a relatively low potency of Taxol productivity (2-10 μg/L).The putative sample spots gave the identical mobility rate and color of the authentic Taxol under UV-illumination at λ 254 nm, were considered, ensuring the chemical identity of the samples as Taxol.The putative Taxol samples was scarped-off from the TLC silica, and dissolved in methanol.The concentration of the putative Taxol samples were confirmed by HPLC, gave a sharp peaks at 4.4 min that was identical to the authentic one.Thus, from the TLC and HPLC, the putative sample was relatively authenticated as Taxol.

Identification of the most potent Taxol-producing fungi, and PCR mining of bapt
The morphological characteristics of the most potent Taxol-producing fungal endophyte of E. whitelockii, was observed by growing on PDA and Czapek's-Dox agar media along for 10 days at 30 ℃, and their macro and microscopical traits were checked daily.The developed fungal colonies obviously appeared whitish, then turned to black after 4 days, the sides of the colonies appear pale yellow producing radiating fissures.The fungus has smooth colored conidiophores, radial biseriate conidial heads, with conidial size 3.5-5.0mm and vesicle 45-80 μm (Fig. 2).The morphological identity of the current fungal isolate completely follow the descriptions of A. niger as described by Samson et al. [56] and Raper and Fennel, [39] The morphological identity of the most potent isolate "A.niger" was verified from the molecular sequence of their rRNA region.The genomic DNA was extracted, used as a PCR template, and the resulting amplicon was ~ 500-520 bp (Fig. 2).The PCR product was purified, sequenced, and non-redundantly BLAST searched on Genbank.From the alignment analysis on the NCBI, the retrieved sequence was displayed 100% similarity with the isolates of A. niger with zero E-value.The sequence of ITS of A. niger EFBL-AG was deposited into the Genbank at accession # OR414905.1.The phylogenetic relatedness of A. niger was constructed by alignment with the data based deposited ITS sequences of A. niger.The isolate A. niger EFBL-AG OR414905.1,an endophyte of E. whitelockii, had a 99.5% identity with the isolates of A. niger with accession # MH341159.1,KP172477.1,MF078659.1,KJ881377.1,LC496501.1,KU171053.1,MW282896.1,MH279840.1,and KJ881376.1 with zero E-value and query coverage (98-100%).
The metabolic potency of Taxol biosynthesis of A. niger has been assessed based on the expression of 10-deacetyl-baccatin III-O-acetyltransferase, as a rate-limiting gene of Taxol biosynthesis.From the PCR results, an amplicon of bapt was ~ 450 bp for A. niger, the amplicon was sequenced (Fig. 2).The sequence of bapt was in silico translated by ExPASy portal tool (https:// www.ebi.ac.uk/ Tools/ st/ emboss_ sixpa ck).The phylogenetic analysis of the BAPT protein was constructed by MEGA X software package (Fig. 2

Chemical identity of the extracted Taxol from A. niger
The chemical identity of Taxol extracted from A. niger was verified by UV-Vis, FT-IR, and LC-MS/MS Analyses.Based on the UV-absorption spectral analysis, the purified Taxol of A. niger exhibited a maximum absorption peak at 227 nm, that was consistent with the standard absorption spectrum of authentic Taxol.From the FT-IR spectra, the purified Taxol had a broad peak in the range of 3475.04 to 3290.39 cm -1 owing to the stretching of hydroxyl (OH) group and amide (-NH) group, as well as, to the stretching of the aliphatic CH groups in the area of 2976 to 2853 cm -1 .The peaks at 1734.06 and 1601.65 were designated to the ester group and aromatic rings stretches, respectively.The COO stretching frequency peaked at 1259.69 cm -1 , while the alkyl C-O stretching of ester appeared at 1073.10 cm -1 .The Aromatic C and H bends frequency peaked at 1020.01 cm -1 as shown in Fig. 3.
The chemical structure of Taxol was resolved by UPLC-ESI-MS/MS in a positive mode.The LC-MS analysis revealed that the Taxol sample had a molecular mass to charge ratio of 854.5 m/z (Fig. 3), that was similar to authentic Taxol from Taxus brevifolia [57].The molecular identity of Taxol has been further resolved from the LC-MS/MS analysis, the parent molecule of 854.5 m/z was further fragmented, displayed a similar fragmentation pattern of authentic Taxol [58].The mass fragmentation of the parent Taxol molecule was illustrated in Fig. 3.

Antiproliferative and antifungal activity activities of ethyl acetate extract and purified Taxol from A. niger
The antiproliferative activity of ethyl acetate extract and purified Taxol from A. niger was evaluated against human hepatocellular (Hep-G2), breast (MCF-7) and intestinal (Caco-2) carcinoma cells, compared to the normal Vero cells.The fungus was grown on PDB medium, incubated at the desired conditions, the culture filtrate was extracted with ethyl acetate, and Taxol was fractionated by TLC.The putative Taxol spots with the same mobility rate and color of the authentic one were scraped off and Taxol was eluted, and their activity was evaluated against the experimental cancer cell lines.From the viability of cells (Fig. 4), the ethyl acetate extract of A. niger showed a significant effect against MCF-7 (IC 50 value 8.9 μg/mL), Hep-G2 (IC 50 value 15.36 µg/mL) and Caco-2 (IC 50 value 68.9 μg/ mL), compared to the normal Vero cells.The selectivity index of the ethyl acetate extracts of A. niger towards MCF-7 and HepG-2 cells, were 17.6 and 9.4, respectively.The purified Taxol of A. niger exhibited a significant cytotoxic activity towards the tested cell lines.From the IC 50 values, Taxol had a strong activity against the MCF-7 and HepG2 cells (~ 0.014 μM), and Caco-2 cells (0.067 μM), with selectivity index about 22.2, revealing the efficiency and specificity of Taxol in targeting the tumor cells than normal Vero cells.
The antifungal activity of the ethyl acetate extracts of the recovered fungal isolates from E. whitelockii was assessed by well-diffusion assay, towards Aspergillus flavus, as a model human fungal pathogen.From the data (Fig. 4), obviously the positive Taxol-producing endophytic fungal isolates have a remarkable activity against A. flavus, in a concentration-dependent manner.The ethyl acetate extracts of A. niger had the highest activity against A. flavus, as revealed from the diameter of the inhibition zone (30 mm) followed by A. awamori (23 mm), and A. candidus and A. fumigatus (17-18 mm) at 10 μg/mL.Practically, the putative Taxol sample exhibited a substantial activity against A. flavus in a concentration-dependent manner, in contrast to the absence of such activity against A. niger as a producer strain (Fig. 4).The lack of biological activity of the extracted Taxol on A. niger, confirm the possessing of a specific resistance mechanism to Taxol that might be by blocking the receptors on the surface of cell membrane or by re-orienting the molecular stereo-structure of tubulin proteins, to be inaccessible for binding with Taxol.

Anti-Wound healing activity of the cells due to the purified Taxol of A. niger
The effect of purified Taxol of A. niger on the migration of the HepG2 and MCF-7 cells, was assessed by measuring the wound closure after 24 and 72 h, compared to the untreated cells.The results showed that the wound closure of HepG-2 and MCF-7 monolayer cells was remarkably suppressed by the purified Taxol, compared to the negative control (Fig. 5).The wound healing percentage of the homogenous monolayer of HepG2 and MCF-7 was approximated by 49% and 54%, after 24 h, and by 52% and 59% after 72 h, respectively, compared to the untreated cells.The suppression of wound healing of HepG2 and MCF-7 cells upon addition of the purified Taxol of A. niger, confirmed the interference with the cellular regeneration, and matrix formation of tumor cells, ultimately halts their metastasis.

Apoptosis and cell cycle analysis of the MCF7 responsive to the purified Taxol of A. niger
The effect of the purified Taxol of A. niger on the apoptosis of MCF-7 cells was assessed by Annexin V-propidium iodide assay.From the results (Fig. 6), a significant shift was observed for the cells to an early apoptotic and late apoptotic stages, due to Taxol of A. niger, compared to the control cells.The MCF-7 cells percentage in early, late apoptosis, and necrosis were about 15.2%, 4.6% and 3.9%, in response to Taxol of A. niger, while, the early apoptosis, late apoptosis and necrosis were represented by about 2.71, 0.71 and 1.78%, respectively, in control cells.The percentage of total apoptosis of the MCF-7 cells was increased by about 11 folds in response to Taxol treatment, compared to the control cells.
The MCF-7 cell cycle with Taxol treatment was analyzed by propidium iodide, the cells were amended with purified Taxol of A. niger, at their IC 25 values, after incubation, the cells were harvested by centrifugation, fixed by 1 mL ice cold 70% ethanol for 2 h at 4 ℃.The DNA content of the cells was analyzed and the ratios of G0-G1, S and G2-M cells were determined.From the results (Fig. 6), the maximum growth arrest of MCF-7 cells in response to Taxol treatment was reported at the G2/M values, compared to the control cells.However, the purified Taxol had no obvious effect on the S-phase

Bioprocess optimization of Taxol production of A. niger by the plackett-burman and central composite designs (CCD)
The productivity of Taxol by A. niger, an endophyte of E. whitelockii, was maximized by optimizing the fungal nutritional requirements.The medium constituents and their interactions are crucial in regulating the biosynthetic machinery of fungal secondary bioactive metabolites.The Response Surface Methodology has been frequently employed to nutritionally optimize the fungal growth to maximize their secondary metabolites yield [44]. Nineteen variables including carbon, nitrogen precursors, growth modulators, and elicitors were evaluated.The lowest and highest values of Plackett-Burman design of each parameter were summarized in (Table 1).After fungal growth, Taxol was extracted, quantified, and the significance of the independent variables affecting Taxol productivity by A. niger, along with the predicted and actual responses from the matrix of Plackett-Burman design were summarized (Table 2).All experiments were conducted in triplicate.Statistical analysis    of this design illustrated the significance of this Model with F-value of 9.04.There is only 0.05% noise of this as revealed from the F-Value.Also, the model terms hold significant importance when the values of "Prob > F" are less than 0.0500.The "Predicted R-Squared" of 0.5174 is in rational agreement with the "Adj.R-Squared" of 0.6791."Adeq.Precision" signal to noise ratio was about 10.011 revealing the adequacy of the signal (Table 3).The plotting of the main effects and normal probability of the tested variables were assessed (Fig. 7), demonstrating that there were five different independent variables, S-methyl jasmonate (X15), T-Ammonium sulfate (X14), B-Lactose (X4), A-Maltose (X10) and R-Fluconazole (X13), had a significant effect on Taxol productivity by A. niger.The significance of the factors on Taxol productivity by A. niger, as well as the actual and predicted yield of Taxol, can be observed through the Pareto chart and probability plots of the independent variables (Fig. 7).
The evaluation of statistical significance of each variable was conducted using the p-value and student's t-test, as shown in Table 3.The distribution of residuals around the diagonal line confirmed the independent normality of the variables, indicating a precise alignment between the predicted and actual Taxol yield The highest actual (267.16µg/L) and predicted (233.5 µg/L) yields of Taxol by A. niger was reported at Run #7, with the Plackett-Burman design, with about 1.5 folds increases in Taxol yield of A. niger, compared to the control "non-optimized" fungal cultures (173.9 µg/L).
The most significant parameters affecting Taxol production by A. niger as revealed from the Plackett-Burman design including maltose, lactose, fluconazole, methyl-jasmonete and ammonium sulfate were further optimized by Central Composite Design (CCD).The interactions of the five parameters "maltose, lactose, fluconazole, methyl-jasmonate and ammonium sulfate" were tested at five levels, to assess their physiological interactions of on Taxol production A. niger.From the CCD results (Table 4), the maximum productivity of Taxol (279.8 µg/L) by A. niger with the CCD bioprocessing was recorded at the run #7, i.e. by about 1.1 folds increments over the Plackett-Burman deisgn.The highest Taxol productivity by A. niger upon CCD, was achieved at 4 g/l maltose, 4 g/L lactose, 1.0 g/L fluconazole, 0.2 g/l methyl-jasmonate and 2 g/L ammonium sulfate incubated for 15 days.The factors with p-value < 0.1 were considered to be significant.The most significant factors affecting Taxol production was reported at 90% confidence, with r2 value 97.8%, revealing the goodness of fit of the regression model.Thus, the interaction between maltose, lactose, fluconazole, and methyl-jasmonate were the most significant factors affecting Taxol production by A. niger.

Metabolic stability of Taxol production by A. niger with the storage and effect of the plant leaf extracts on restoring the Taxol productivity
The influence of storage of A. niger at 4 ℃ along 10 months was assessed on their Taxol biosynthetic potency, compared to the zero culture.After incubation of each culture, Taxol was extracted and quantified by TLC and HPLC.A noticeable relative stability of Taxol biosynthetic machinery has been observed till 6 months of storage as slope cultures at 4 ℃ (Fig. 8).The productivity of Taxol by A. niger was attenuated by about 1.25 folds after the 6 months (180.7 μg/L) of storage, compared to the first culture (234.43 μg/L).The yield of Taxol by A. niger was reduced by about 2.6 folds at the 10th month of storage (91.8 μg/L).Thus, from the linear equation of Taxol productivity by A. niger, the biological half-life time of metabolic biosynthetic stability of Taxol was approximated by about 6.1 months as slope cultures at 4 ℃.As well as, the mycelial melanin pigmentation of A. niger was obviously faded with the fungal storage, revealing the apparent connection with the expression of the biosynthetic genes of polyketides with the storage time, that might be correlated with the Taxol reduction over the time [21].
The effect of organic leaves extracts of E. whitelockii on restoring the Taxol biosynthetic potency of A. niger has been assessed.The extracts of dichloromethane, ethyl acetate and ethanol of E. whitelockii leaves, was amended to 5 days pre-culture of the zero culture and 8 months stored A. niger, then completed to 15 days of incubation, Taxol was extracted and quantified.From the obtained results (Fig. 8), the ethyl acetate extracts of E. whitelockii displayed a slight inducing effect of Taxol biosynthesis of 8 months stored culture of A. niger by about 1.3 folds (210 μg/L), however, this extract had no obvious effect on Taxol production by the zero culture of A. niger.The extracts of dichloromethane and ethanol had no noticeable effect on induction of Taxol biosynthesis by the stored A. niger culture.The remarkable inducing effect of ethyl acetate extracts, compared to other extracts, might be due to the high polarity of the ethyl acetate extracting unique compounds, than the other solvents.

Discussion
Despite the fungal fast growth, resistance to mechanical stress, and feasibility of genetic manipulation, the commercial production of Taxol by fungi has been halted by the rapid loss of Taxol productivity with the storage and subculturing [14,18,22,59].Thus, screening for a novel endophytic fungal isolate inhabiting the plants of ethnopharmacological uses, could have relatively stable Taxolproducing machinery.From literature, the biological and metabolic identities of the fungal endophytes inhabiting E. whitelockii remains equivocal, thus, mining of fungal endophyte with metabolically stable Taxol-producing machinery with the storage was the main objective of this study.Eight fungal isolates were recovered from the leaves of E. whitelockii, the highest Taxol producing potency was reported for A. niger (173.9 μg/L).The isolate of A. niger was morphologically identified based on the descriptions of Samson et al. [56] and Rapper ad Fennel et al. [39].The isolate of A. niger was molecularly identified based on its internal transcribed spacer (ITS) sequence, the sequence was deposited on Genbank with accession number OR414905.1.This is the first report describing isolate of A. niger as an endophyte of E. whitelockii, that has the Taxol producing potency.Similar results reported the Taxol producing potency from gymnosperms; Ginkgo biloba [44] and Podocarpus gracilior [21].The Taxol yield by A. niger has been matched with Penicillium polonicum [44], A. flavipes [60], A. terreus [21], endophytes of Podocarpus gracilior, as well as endophytes of Taxus sp such as A. niger [61], Fusarium solani [62] and A. candidus [63].The metabolic potency of Taxol production was verified by the PCR mining of 10-deacetyl-baccatin III-O-acetyltransferase as rate-limiting gene of Taxol biosynthesis.Molecular verification of Taxol biosynthesis via PCR mining of the signature genes encoding the rate-limiting enzymes was recognized as preliminary marker for assessing the molecular blueprint of Taxol biosynthesis [21,64,65].The chemical identity of the purified Taxol from A. niger was confirmed by HPLC, UV-absorption spectra, FT-IR spectra, and LC-MS/MS.Collectively, from these spectral and chromatographic analysis, the purified putative Taxol from A. niger had the same spectral and chemical structures of the authentic Taxol from T. brevifolia [14].Additionally, the putative Taxol sample from A. niger had a molecular mass to charge ratio of 854.5 m/z, which was identical to the authentic Taxol from T. brevifolia [14].Furthermore, the parent molecule of the putative Taxol from A. niger exhibited the same molecular fragmentation pattern as the authentic Taxol, confirming their chemical identity as Taxol [20,58,66].
The antiproliferative activity of ethyl acetate extract and Taxol from A. niger was evaluated against human hepatocellular (Hep-G2), breast (MCF-7) and intestinal (Caco-2) carcinoma cells compared to the Vero cells.From the IC 50 values, the ethyl acetate extract of A. niger had a significant effect against MCF-7 (8.9 μg/mL), Hep-G2 (15.36 µg/mL), with obviously tiny activity to the Vero cells.The purified Taxol of A. niger had a significant activity towards the MCF-7 and HepG2 cells (~ 0.014 μM), and Caco-2 cells (0.067 μM), with selectivity index 22.2, revealing the efficiency and specificity of Taxol in targeting the tumor cells than normal ones.According to the National Cancer Institute (NCI) reports, the potent cytotoxic compounds should be of IC 50 value < 20 μg/mL [67].
Ethyl acetate extract was found to have a potent cytotoxic activity with IC 50 of < 20 μg/mL on MCF-7 and Hep-G2, that being similar with the ethyl acetate extracts of A. flavus, with IC 50 for MCF-7 cells of 16.25 µg/mL [68].
A. flavus is the second most prevalent opportunistic pathogen that causes both invasive and superficial infections in humans, exhibiting the highest levels of morbidity and mortality [69].Ethyl acetate extract of A. niger of E. whitelockii exhibited superior antifungal activity than ethyl acetate extract of A. niger, an endophyte of Alangium salviifolium, or other host plants against A. flavus [70,71].This finding could be attributed to the fluctuation in the metabolite contents of fungal endophytes in relation to different host plants [22].Taxol purified from A. niger has superior antiproliferative activity to Hep-G2 and MCF-7 tumor cells than P. polonicum Taxol (IC 50 values of 4.06 μM and 6.07 μM) [44].The antiproliferative activity of A. niger extracted Taxol towards the various human carcinoma cell lines were closely similar to Taxol purified from various fungal sources [20,[72][73][74][75]. Taxol inhibits the formation of functional spindles during metaphase in mitosis and cellular proliferation, disrupting the normal reorganization of the microtubules, ultimately leads to the induction of apoptosis and cell mortality during the G2/M phase of cell cycle [76][77][78].Obviously, the Taxol-producing endophytic fungi have a remarkable activity against A. flavus, in a concentration-dependent manner.The putative Taxol sample has a significant activity against A. flavus in a concentration dependent manner.The lack of activity of the extracted Taxol on A. niger, the same Taxol-producing fungal isolate, confirm the possessing of a specific resistance mechanism to Taxol that might be by blocking the receptors on the cell membrane or re-orienting the molecular stereo-structure of tubulins, to be inaccessible for binding with Taxol.Similar results for dual antiproliferative and antifungal activities of Taxol were reported [79].
The effect of purified Taxol of A. niger on the migration of the HepG2 and MCF-7 cells, was evaluated by measuring the wound closure.The wound healing of the HepG2 and MCF-7 was estimated by 71% and 79% after 72 h, compared to the control.The plausible suppression of healing of tumor cells with the purified A. niger Taxol, confirmed the interference with the cellular divisions, and matrix formation, with ultimate halting of their metastasis.Taxol was extensively researched for its ability to act as an anti-angiogenic agent, hinder wound healing, and impede cell migration across various lines [80,81].Taxol inhibited the human umbilical vein endothelial cells migration, decreasing the length of microtubules; reducing the peripheral microtubules [80].The effect of the purified Taxol of A. niger on the apoptosis of MCF-7 cells was assessed, a significant shift has been observed for the normal cells to apoptotic phase, responsive to Taxol, compared to control.The percentage of total apoptosis of the MCF-7 cells was increased by about 11 folds in response to A. niger Taxol, compared to the control cells.The cell cycle of MCF-7 in response to Taxol treatment was analyzed with the purified Taxol at IC 25 values.The maximum growth arrest of MCF-7 cells in response to Taxol was reported at the G2/M values, compared to the control cells, that being similar with previous results [82].The same biological effect of A. niger Taxol on cell cycle, antiproliferative activity, and cell migration of tumor cells with the Taxol from T. brevfolia, ensures the identical molecular stereo-structure, orientation and structure activity relationships of the purified sample with the authentic one.
The productivity of Taxol by A. niger, endophyte of E. whitelockii, was maximized by the nutritional optimization with the response surface methodology.The significant variables affecting Taxol productivity by A. niger was methyl jasmonate, ammonium sulfate, lactose, maltose and fluconazole.The actual yield of Taxol by A. niger was increased by ~ 2.3 folds, compared to the control cultures.Similar paradigm of maximizing the yield of bioactive metabolites by fungi via nutritional optimization with the Plackett-Burman Design was reported, considering the interactions of the components in regulating the biosynthetic machinery of secondary metabolites in fungi [17,44,50,66].Similar optimization protocol has been employed for maximizing to the Taxol productivity by P. polonicum and A. terreus [21,44].Methyl jasmonate has been recognized a significant elicitor triggering the crosstalk of the plasma membrane receptors, stimulating the plant defense responses, by generating the reactive oxygen species, induce the overexpression of the secondary metabolites [83].Actually, the Taxol yield by A. niger, an endophyte of E. whitelockii, was similar to those of A. terreus, endophyte of Podocarpus gracilior (265.4 μg/L) [20,21], A. niger, endophyte of Taxus cuspidata (273 μg/L) [61], A. candidus, endophyte of Taxus media (112 μg/L) [15], and A. fumigatus, endophyte of Podocarpus sp [3].However, the Taxol yield by the current isolate of A. niger was higher than those reported by F. proliferatum (240 ng/L) and Colletotrichum gloeosporioides (120 ng/L), endophytes of Taxus cuspidata [63].
The Taxol productivity of A. niger preserved as slope culture at 4 ℃ along 10 months was assessed, a relative stability of Taxol biosynthetic machinery has been observed till 6 months.The Taxol productivity by A. niger was attenuated by ~ 1.25 folds after 6 months of storage as slope culture, compared to the first culture, thus, the biological half-life time of metabolic biosynthetic stability of Taxol was approximated by 6.1 months as slope culture.Attenuation of the Taxol productivity by fungi has been reported as one of the most challenges that halt the further implementation of fungi to be an industrial platform for Taxol production [4,17,18,20,21,44].Similarly, the Taxol biosynthetic stability by A. flavipes was reduced by 2 folds by the 6th month storage (135 μg/L) [16].The yield of Taxol by A. terreus was drastically reduced by 4 folds (78.2 μg/L) by the 6th month of storage, compared to the 1st culture [20,21], that being lower than the biosynthetic stability of Taxol by current isolate of A. niger, at the same conditions (174 μg/L).
The ethyl acetate extracts of E. whitelockii displayed a slight inducing effect on Taxol biosynthesis of 8 months stored culture of A. niger by about 1.3 folds (210 μg/L).The remarkable inducing effect of ethyl acetate extracts might be attributed to the higher polarity of the ethyl acetate extracting unique compounds, than the other solvents.So, the Taxol biosynthetic machinery of A. niger may be influenced by chemical signals from the host plant or its associated microbial flora [15,20].
In conclusion, A. niger, an endophyte of E. whitelockii, had the most promising yield of Taxol with relative metabolic stability till the 6th month of storage.The chemical identity of purified A. niger Taxol was confirmed by the FT-IR, HPLC and LC-MS/MS analysis, compared to the authentic one.The purified Taxol of A. niger displayed a significant antiproliferative activity, strong inhibitory effect on tumor cells migration, arresting the cell cycle at the G2/M phase.The biological half-life time of Taxol productivity by A. niger was about 6 months as slope culture at 4 ℃, with partial restoring to the metabolic potency of Taxol productivity by A. niger upon addition of ethyl acetate plant extract, ensuring possessing of some plant-derived signals that triggers the cryptic Taxol encoding genes.Metabolic characterization of the chemical identity of the plant-derived signals, and their effect on restoring the molecular expression of the Taxol biosynthetic machinery by the fungus, are the ongoing studies by our lab.

Fig. 1
Fig. 1 Isolation and screening for the endophytic fungal isolates of E. whitelockii leaves.A Morphological view of E. whitelockii.B Plate cultures of the recovered fungal endophytes.(1.Penicillium citrinum, 2. Fusarium solani, 3. Aspergillus niger, 4. A. awamori, 5. A. candidus, 6. Cladosporium sp., 7.A. flavus and 8. A.fumigaus).C The TLC plate of the extracted Taxol from the fungal isolates normalizing to authentic Taxol.D Yield of Taxol as quantified from the TLC chromatograms.E HPLC chromatogram of the TLC purified Taxol from A. niger, and F. solani, compared to the authentic Taxol at retention time 4.4 min.The yield of Taxol from the HPLC chromatograms for A. niger and F. solani were about 173 and 55 μg/L, that being matched with the TLC analysis

Fig. 2 Fig. 3
Fig. 2 Morphological and molecular identification of the potent Taxol producing fungus.A Plate culture of A. niger on PDA medium after 6 days.B Conidial heads of A. niger with an oval shape, and biserriate strigma at 1000 × magnification.C PCR amplicon of the ITS region of A. niger.D PCR amplicon of bapt gene as Taxol rate-limiting gene of Taxol biosynthesis.E Phylogenetic relatedness of the ITS region of A. niger by the maximum neighbor-joining (NJ) model by the MEGA X software.F The phylogenetic relatedness of the sequence of putative protein sequence of the BAPT retrieved from translation of the bapt gene by the maximum neighbor-joining (NJ) model by the MEGA X.The DNA ladder was 1 kb (Nex-gene Ladder, Puregene, Cat.# PG010-55DI)

Fig. 4
Fig. 4 The antiproliferative and antifungal activities of the extracted Taxol and crude ethylacetate extracts of A. niger.A, The activity of the putative Taxol A, and crude ethylacetate extracts (B) towards the tumor cell lines HepG-2, MCF-7, Caco-2, compared to the normal Vero cells, a revealed from the IC 50 values.C The antifungal activity of the extracted Taxol and crude ethylacetate extracts against A. flavus at concentrations 0.1, 0.5, 2.5, 5 and 10 μg/mL.D The diameter of the inhibition zones of the tested fungal extracts against A. flavus

Fig. 5
Fig.5 Wound healing assay of the HepG2 and MCF-7 cells in response to the purified Taxol of A. niger comparing to the untreated cell lines (negative control), after 24 ad 72 h.After 24 h of growth of the cells as homogenous monolayer, a scratch was made and the extracted Taxol was added (IC50 values 0.02 μM), to the medium, then the wound healing was measured at zero time and after 24 h and 72 h of incubation for the cell lines HepG-2 (A) and MCF-7 (B).The percentage of wound healing of the HepG-2 (C) and MCF7 (D) cells in response to Taxol treatment

Fig. 6
Fig. 6 Flow cytometric apoptotic analysis of the MCF-7 cells by Annexin V-FITC.The cells were exposed to IC25 concentration of Taxol, the apoptosis was measured after 48 h of incubation.Apoptotic analysis of MCF-7 -cells without treatment (A), and in response to treatment with Taxol (B), and the overall quantitative results of apoptosis (C).The cell cycle analysis of the MCF-7 without treatment (D), and in response to treatment with Taxol (E) and overall quantitative analysis of cell cycle (F) . The initial polynomial equation for Taxol production by A. niger reflecting the significant independent variables was represented by the following: Taxol productivity = 65.49333− 8.865 * Maltose + 9.46 * Lactose − 16.48 * Fluconazole + 60.4 * Methyl jasmonate + 16.95333* Ammonium sulphate.

Fig. 7
Fig. 7 The impacts of several variables on Taxol production corresponding to the Plackett-Burman experimental design.The normal probability plots of the variables for Taxol production by A. niger as determined by the first order polynomial equation.A: The Pareto chart displays the significance of all variables.The half -Normal plot (B), The Box-Cox power transform (C), and the normal plot of the standardized effect with normal possibility (D).E Plot of correlation between predicted and actual Taxol yield of. A. niger.F Plot of the standardized effect with normal residuals

Fig. 8
Fig. 8 Biosynthetic stability of Taxol by A. niger in response to storage and effect of plant extracts on restoring its biosynthetic Taxol machinery.A The productivity of Taxol for A. niger in response to fungal preservation as slope culture (The upper panel is the TLC, the lower panel is the yield of Taxol).B The yield of Taxol by A. niger in response to amendment with different extracts of E. whitelockii (The upper panel is the TLC, the lower panel is the yield of Taxol)

Table 1
Lowest and highest values of the selected parameters in Plackett-Burman Design for optimization of Taxol production

Table 2
The Plackett-Burman experimental design matrix for Taxol production by A.niger

Table 3
Analysis of regression statistics and variance ANOVA for Placket-Burman design

Table 4
Matrix and responses of the CCD for the significant factors affecting Taxol production by A. niger